Table 1 |
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Primers used to amplify RACE products and characterize Rx polymorphism. |
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Gene |
Procedure |
Name |
Sequence 5' → 3' |
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|
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hb |
5'-RACE |
F1 |
CAGGCGAAGTCGAACTACGTTCACGACATACC |
|
F2 |
CGCAA-TACCCCGATGTGTTCACGAGAGAGG |
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|
3'-RACE |
R1 |
TCTTGCCTCGCT-CAAGTCCAGCCGAAG |
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|
R2 |
GGGCAAGTTCCTCTCTCGTGAACACATCGG |
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|
otp |
5'-RACE |
R1 |
CGCTGCCAGCTCTTCTCGCATGAACAC |
|
R2 |
CGGA-TAGTGAGTCCTGGCGAAACATCGC |
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|
3'-RACE |
F1 |
CGAGCGATGTTT-CGCCAGGACTCACTATC |
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|
F2 |
CGGATGTGTTCATGCGAGAAGAGCTGG |
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|
rx |
5'-RACE |
R1 |
TGTACCCTAACTTCAGGGAGGCTTATTTTTAGCG |
|
R2 |
TGGATAGTGGGATTTCTCGAAGGCTCGC |
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3'-RACE |
F1 |
TCAGAAGA-AACCGTACCACCTTCACAACG |
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F2 |
TCGAGCGAGCCTTCGAGAAATCCC |
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polymorphism |
RX_for |
GATCCTTAATTCAAACCCTGGGAC |
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RX_rev |
TCGGGCAAATCTGA TACGAGTAG |
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|
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RACE = rapid amplification of cDNA ends. |
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Mazza et al. EvoDevo 2010 1:3 doi:10.1186/2041-9139-1-3 |
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